If the interface inverts polarity you can tick the Invert box in the REW soundcard preferences input options. A loopback measurement will show whether there is inversion, by looking at the impulse response with percent as the Y axis to see if it is the right way up. Monitoring may be controlled by the interface or its mixer software, on some interfaces there is a mixer knob of some form with input at one end and playback or DAW at the other. If there is such a knob it should be all the way around to DAW/playback. If monitoring is active that will also show up in a loopback measurement, as shown in step 5 of interface calibration.
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